Methods Epithelial cells were isolated from sample tissue by cold trypsinization and cultured in serum-free growth medium.

方法 标本组织块用冷消化法分离出尿路上皮细胞，培养于无血清生长培养基。

Interactive effects of growth medium, cryoprotector and starvation treatment on bacterial survival during the storage of freeze-dried lactic acid bacteria have been studied.

研究了培养基、冻干保护剂、饥饿处理对冻干乳酸菌存活性的交互影响。