Would you be willing to stake your life on it?

你愿以生命作赌注吗？

The expression " to stake (stake) your life on something" means to be completely sure of something, so sure that you would die if you were wrong.

“to stake (stake) your life on something” 这个表达的意思是对某事完全确定， 确信如果你错了你会死。