单词 | agar |
例句 | We can imagine them, curious and dispassionate, observing us, as we would watch a bacterial culture in a dish of agar, to determine whether, this year again, we manage to avoid self- destruction. Cosmos 1980-01-01T00:00:00Z Silence is in the military's cultural agar, and you would be hard-pressed to find a more politically circumspect population than the tight-knit community of spouses. Don’t speak for my military family: A veteran’s wife on Colin Kaepernick and the Nike boycott 2018-09-05T04:00:00Z There's even a product called agar gel, which is surprisingly quite firm! Everything you should know about agar-agar — and how to cook with it 2021-07-07T04:00:00Z Agar agar can be used as a substitute if you're trying to avoid gelatin, and it should be handled in the same way. How to make mousse, according to Food52's resident baker 2022-01-13T05:00:00Z Simply made from bean paste, sugar and the thickening agent agar, it’s usually formed into chewy, colorful little blocks, sometimes flavored with banana or coconut. A Japanese Sweet Tradition a Thousand Years in the Making 2019-11-04T05:00:00Z Wine jelly can be made using agar agar, a seaweed-based substitute for gelatin commonly used by vegans and vegetarians. Wine jelly is the nostalgic dessert that deserves a comeback 2022-05-29T04:00:00Z There will be a diorama lined with tile and containing sculptures coated with agar, a gelatinous substance that supports the growth of bacteria gathered in Manhattan’s Chinatown and Koreatown. The Beauty of Bacteria: Anicka Yi’s ‘Life Is Cheap’ 2017-04-15T04:00:00Z But when you reach it, look down and you’ll see a rectangular slab of agar on which the show’s title is faintly blooming, painted with the bacteria. Scent of a Woman 2015-04-03T04:00:00Z You use it the same way you would gelatin, too: Dissolve and hydrate the agar in warm liquid and let set. Everything you should know about agar-agar — and how to cook with it 2021-07-07T04:00:00Z Each has a different scent, which include jasmine, cinnamon, sandalwood, citrus and agar wood. A ‘Plant-Based’ Gift Guide 2021-11-23T05:00:00Z Bear in mind that gelatin is made by cooking animal bones to release their collagen, so vegetarians and vegans may instead want to use powdered agar, a type of seaweed. Gelatin Breaks Out of Its Mold 2018-11-09T05:00:00Z Instead of using gelatin, recipe developer Amy Chaplin had the super smart idea to utilize agar agar flakes for the fruity filling in this vegan tart recipe. 17 vegan pie recipes that are practically perfect 2021-09-28T04:00:00Z But do not imagine you are sitting in an agar plate of vicious microbes every time you belt up. How dangerous are the bugs in my dirty car? 2015-08-10T04:00:00Z Full disclosure, I've never used this substitution, but I have a friend who made it with agar agar, and it turned out just fine. Wine jelly is the nostalgic dessert that deserves a comeback 2022-05-29T04:00:00Z Microbiologists from Nottingham University took swabs from steering wheels, foot wells and seats and put them on agar plates to see what grew. How dangerous are the bugs in my dirty car? 2015-08-10T04:00:00Z Hesse, who was married to a German scientist, suggested growing microbes in agar, which her grandmother used in pudding recipes. Your Body Is a Wonderland 2019-10-15T04:00:00Z The gummy section covers various gelling agents like gelatin, agar agar, pectin and tapioca starch. Meet the cookbook author who's bringing Taiwanese-American flavors to cannabis edibles 2022-09-17T04:00:00Z The bacteria was spread on agar jelly and placed under glass, forming a portrait about A4 size. When two tribes meet: collaborations between artists and scientists 2011-08-21T20:30:01Z She seems to have thrived in the agar of generous narcissism that her parents exuded, knowing how to draw what she needed. Literary Lions, by Their Cubs 2011-08-10T21:14:41Z The Terrazzo Coffee Jelly, made with agar agar, pays homage to the version my mom used to make for potluck parties. Meet the cookbook author who's bringing Taiwanese-American flavors to cannabis edibles 2022-09-17T04:00:00Z Petri dishes of agar affixed to the cardboard family, which was positioned at different distances of up to three metres, captured and revealed any dispersed viruses. Hand dryers v paper towels: the surprisingly dirty fight for the right to dry your hands 2019-04-25T04:00:00Z Limoncello “pearls” created with the help of lemon and agar agar. Review | Tom Sietsema’s 6 favorite places to eat right now 2023-01-24T05:00:00Z Chloe swabs parts of her body, or plants, and transfers the samples to petri dishes with a special growth medium inside called agar. TikTok designer creates jewellery from bacteria 2022-10-03T04:00:00Z First, they put dozens of different strains of drug-resistant pathogens onto agar plates containing 5 percent sheep blood and incubated them overnight. A drug that can kill antibiotic-resistant bacteria could stave off the coming superbug crisis 2022-09-19T04:00:00Z Figure 22.7 In these agar plates, the growth medium is supplemented with red blood cells. Biology for AP Courses 2022-06-09T00:00:00Z The patterns in this image, called Fractals, are of Bacillus subtilis being grown on a dextrose agar plate. Ants feeding off honeydew wins the Royal Society of Biology's photo prize 2021-10-07T04:00:00Z Recipes vary, but Lim mixed ingredients like agar, soy flour, malt extract, yeast, molasses and cornmeal. How to Breed Fruit Flies 2021-08-17T04:00:00Z I ordered some agar, a seaweed-based gelling agent, and tried the most common vegan versions of marshmallows. This recipe for vegan marshmallows uses just three ingredients 2021-02-23T05:00:00Z It now appears that the coronavirus — and the concomitant social and public health measures required to stem its spread — served as the perfect agar plate to culture a new round of pandemic-related misinformation. We asked experts to respond to the most common COVID-19 conspiracy theories 2020-07-18T04:00:00Z Blood agar becomes transparent in the presence of hemolytic Streptococcus, which destroys red blood cells and is used to diagnose Streptococcus infections. Biology for AP Courses 2022-06-09T00:00:00Z The bacteria were delivered alive, teeming on a medium of agar made from Dorset egg yolks and sealed with paraffin wax. A Living Library Filled With Killer Bacteria 2020-06-08T04:00:00Z The researchers fill the holes with a microbial sample diluted in molten agar, in the hope that each chamber will contain one or a few starter microbes. The search for microbial dark matter 2020-06-07T04:00:00Z The embryos, which resemble tiny clusters of pale, translucent grapes, live in agar, a puddinglike substance derived from algae, in petri dishes. Can Genetic Engineering Bring Back the American Chestnut? 2020-04-30T04:00:00Z To create a strong and stable compound, she added the molecules chitosan from crustaceans and agar from red algae to her scales-and-skin mixture. Tipping the scales? Briton develops fish waste 'plastic' 2019-11-13T05:00:00Z Samples were plated on nutrient agar plates, some of which were supplemented with the antibiotic ampicillin. Biology for AP Courses 2022-06-09T00:00:00Z In the lab, they are fed a standard diet consisting of a slurry of malt, corn meal, yeast, agar and syrup. How Monarch Butterflies Evolved to Eat a Poisonous Plant 2019-10-02T04:00:00Z She tried liquid culture, solid culture and chocolate agar, made from lysed red blood cells, among other recipes. The search for microbial dark matter 2020-06-07T04:00:00Z There will be people among Emerald’s machines, but they will mostly also be following software-generated instructions — shifting and scanning sample vials and agar plates. An operating system for the biology lab 2019-09-24T04:00:00Z Immediately remove from heat and, using an immersion blender, slowly add the agar agar, making sure all is incorporated. Like everything at Tarsan i Jane, these stuffed ice cream sandwiches are anything but stuffy 2019-09-17T04:00:00Z Something called agar agar, which is made from red algae, is often referred to as vegan gelatin and is used in lots of desserts. Percy Pigs: What is gelatin and why do we eat it? 2019-05-01T04:00:00Z Leary mixed these samples together, grew them on plates of agar and then sterilised the mixture. The endless hunt for the perfect flu vaccine 2019-01-24T05:00:00Z “We get entirely new stuff that is entirely different from what you get on an agar plate,” he says. The search for microbial dark matter 2020-06-07T04:00:00Z Leary mixed these samples together, grew them on plates of agar, and then sterilized the mixture. The 1918 Influenza Pandemic: How Far Have We Come? 2018-12-18T05:00:00Z A live tentacle placed on the membrane immediately pierces it and injects venom into the agar; blood cells destroyed by porins show up as white patches against the vibrant red. Jellyfish almost killed this scientist. Now, she wants to save others from their fatal venom 2018-11-08T05:00:00Z The first step is to grow molds in a material such as agar, a jelly-like substance found in some types of seaweed. Mushrooms could help clean toxic groundwater in New Mexico 2018-10-03T04:00:00Z Citric acid, agar agar, iota carrageenan, miso and harissa paste can be found at select cooking stores, as well as online. Culinary SOS: You can build an entire dinner party around this roasted carrot salad - Los Angeles Times 2018-09-28T04:00:00Z Plus, Jogler adds, the agar commonly used as a gelling agent might inhibit growth, so he sometimes tries alternatives such as gellan gum. The search for microbial dark matter 2020-06-07T04:00:00Z She notes that agar, another phycocolloid derived from red seaweeds, is widely used as a growth medium in petri dishes and to separate strands of DNA. Review | An homage to seaweed and a plea for wise management of this valuable resource 2018-08-23T04:00:00Z You can’t tell by eye which bacteria growing within a petri dish are the “good guys” versus ones that cause disease, and some pathogenic microbes, like viruses, can’t be detected within agar petri dishes. You Are Probably Washing Your Hands Wrong 2017-12-17T05:00:00Z Later methods that screened patients for urinary-tract infections by culturing bacteria on slabs of agar jelly reinforced this idea. Microbiome: A bag of surprises 2017-11-07T05:00:00Z Whisk in the agar agar, iota carageenan and citric acid and bring the mixture to a hard boil. Culinary SOS: You can build an entire dinner party around this roasted carrot salad - Los Angeles Times 2018-09-28T04:00:00Z When agar is heat-sterilized together with phosphates, it produces hydrogen peroxide that prevents some microbes from growing. The search for microbial dark matter 2020-06-07T04:00:00Z A silk quilt on display looks cosy, but the vivid squares are patterned with drug-resistant strains of bacteria, such as MRSA, grown on agar containing multicoloured dye. Disaster preparedness: Risk, rout and ruination : Nature : Nature Research 2017-10-24T04:00:00Z In the lab, these worms live on agar plates and are fed a diet of live bacteria. A long journey to reproducible results 2017-08-21T04:00:00Z In one such scientifically-infused creative process, a visual arts major, Louisa Willis, experimented with an overhead projector, growing agar plates of bacteria with the projector’s heat and imaging the plates with the projector’s light. Is It Art or Is It Science? 2017-07-27T04:00:00Z Since this was clearly unwise, we compromised by creating life-size models of my head and body out of a nutrient-rich jelly called agar. Could ants tackle the 'antibiotic apocalypse'? - BBC News 2017-05-17T04:00:00Z The scientists tested various solutions on sheep and human blood cells suspended in agar. Vinegar makes best antidote to jellyfish stings, study finds 2017-05-09T04:00:00Z Growth in soft agar was performed as described in ref. LACTB is a tumour suppressor that modulates lipid metabolism and cell state : Nature : Nature Research 2017-03-21T04:00:00Z Test compounds are mixed into the agar or feedstock, and longevity is assessed by mobility — if a worm moves when prodded with a metal wire, it's alive! A long journey to reproducible results 2017-08-21T04:00:00Z As a postdoc, plant biologist Christopher Topp was not satisfied with the usual way of studying root development: growing plants on agar dishes and placing them on flatbed scanners to measure root lengths and angles. Plant biologists welcome their robot overlords 2017-01-24T05:00:00Z He swabbed my agar face with a multi-drug resistant strain of pseudomonas and then covered part of it with a small bandage, impregnated with phages. Could ants tackle the 'antibiotic apocalypse'? - BBC News 2017-05-17T04:00:00Z The creatures were stuck in one agar dish, and their food was placed in a second. Slime molds prove you don’t need a brain to learn — or teach — a new trick 2016-12-30T05:00:00Z The dish is filled with agar jelly, a nutritious medium perfect for growing bacteria, and its surface is divided into channels containing different concentrations of antibiotic drugs. Watch evolution in action as bacteria mutate to resist antibiotic drugs 2016-09-09T04:00:00Z To the typical medium—a nutrient-rich gelatin called agar, derived from seaweed—they tried adding blood, chicken bouillon, urine. Miracle Microbes? 2016-06-13T04:00:00Z Applying shotgun metagenomic sequencing, we profiled and compared the bacterial species present in the original faecal samples to those that grew as distinct colonies on agar plates containing the complex, broad-range bacteriological medium, YCFA7. Culturing of ‘unculturable’ human microbiota reveals novel taxa and extensive sporulation : Nature : Nature Publishing Group 2016-05-03T04:00:00Z Agar — the seaweed-derived, gelatinous substance that biologists use to culture microbes — is experiencing a global downturn, marine biologists, agar producers and industry analysts told Nature. Lab staple agar hit by seaweed shortage 2015-12-07T05:00:00Z Another upgrade instructs the bacteria to excrete an enzyme to stain the dish’s agar solution black when hit by light. Biohackers push life to the limits with DIY biology 2015-11-18T05:00:00Z The agar man, Hiroshi Tsukakoshi, 77 years old, preaches wariness of American management methods, which he says are warped by short-term thinking. To Whom Do Japan’s Most Powerful Turn for Advice? The Sensei of Seaweed 2015-09-01T04:00:00Z I started playing an online game called agar.io a few weeks ago, and by now it's swallowing up almost all my leisure time. This online blob game has reduced me to a shell of a man 2015-08-16T04:00:00Z They had grown 10,000 types of bacterium — many more than if they had just put the soil sample on an agar plate. Mining the microbial dark matter 2015-06-15T04:00:00Z Throughout the 2000s, the nation regularly harvested as much as 14,000 tonnes per year, which was sold on to foreign and domestic agar producers. Lab staple agar hit by seaweed shortage 2015-12-07T05:00:00Z The talk is not yet online, but I discussed some central points in an interview with Midwest Energy News, one being whether we can do better than bacteria smeared on agar? Scenes from a Sustainability Quest, from Leopard Skin Sales to Lionfish Sashimi 2015-04-03T04:00:00Z The complex houses an agar research and development center, an agar exhibition, an agar factory, two agar shops and two agar restaurants. To Whom Do Japan’s Most Powerful Turn for Advice? The Sensei of Seaweed 2015-09-01T04:00:00Z Using a lattice of tiny wells holding individual bacteria within agar, the iChip is covered with a permeable membrane, and replaced into the original soil. Teixobactin and iChip Promise Hope Against Antibiotic Resistance 2015-01-08T05:00:00Z For the Cell paper, Dutton and her team reconstructed the microbial communities from cheese rinds by adding different combinations of bacteria and fungi to an agar plate made of milk curd. Scientists and cheesemakers gather for (microbial) culture 2014-08-26T04:00:00Z Current agar deficiencies are due mostly to an unsteady supply of Moroccan Gelidium, say industry insiders. Lab staple agar hit by seaweed shortage 2015-12-07T05:00:00Z After the bacteria were chemically treated, samples were put on agar plates and incubated 24 hours. C.D.C. Details Anthrax Scare for Scientists at Facilities 2014-06-19T04:00:00Z On a sunny afternoon in July, tourists were relaxing in the Kanten Papa Garden—named after the company’s best-known brand of kanten, as agar is known in Japanese. To Whom Do Japan’s Most Powerful Turn for Advice? The Sensei of Seaweed 2015-09-01T04:00:00Z It might appear as a kind of wobbly terrine, a chilled consomme or a flavored construction of agar. At Awu Delicious Food in Arcadia, much to try along with your koi jello The TB bacterium is also slow in the lab, where a single bacterium takes three weeks to form a colony on agar plates. A Networked Approach to Fighting the TB Pandemic 2014-03-24T11:30:28Z Millipore Sigma blames the shortage on competition from food companies for purified agar. Lab staple agar hit by seaweed shortage 2015-12-07T05:00:00Z Modern genetic techniques allow these to be sampled far more effectively than by the old approach of culturing on agar plates. Olfactory communication: Spot the difference 2014-02-20T15:58:34Z Mr. Toyoda’s reverence for the agar maker helps explain the stickiness of traditional business values in Japan, where long-term success and social cohesion are often favored over short-term profitability. To Whom Do Japan’s Most Powerful Turn for Advice? The Sensei of Seaweed 2015-09-01T04:00:00Z We are different than bacteria on agar, he contends. Dot Earth Blog: An Ecologist Explains His Contested View of Planetary Limits 2013-09-16T14:02:55Z Koch grew bacteria on agar, a gelatinous substance made by boiling a marine algae. Google celebrates Julius Richard Petri, inventor of the Petri dish 2013-05-31T16:01:10Z Before a technician introduced agar to his lab, pioneering German microbiologist Robert Koch isolated bacteria on potato slices. Lab staple agar hit by seaweed shortage 2015-12-07T05:00:00Z Petri was not the first to use agar, a substance made from algae, to culture bacteria but he invented the standard dish in which it was done. Julius Richard Petri: Google celebrates birth of inventor of the petri dish 2013-05-31T01:30:08Z Nakagaki and his teammates placed blocks of agar packed with nutrients at the start and end of the maze. How Brainless Slime Molds Redefine Intelligence 2012-11-07T16:45:02.660Z They placed the organism, which Mr Reid described as a "large, blob-like cell", on one side of a dish of agar gel. Brainless slime's external memory 2012-10-09T06:45:32Z But there was not a convenient container to hold agar until Petri invented a special dish that then became standard throughout the world. Google celebrates Julius Richard Petri, inventor of the Petri dish 2013-05-31T16:01:10Z Creating any genetically modified plant involves culturing a blob of plant cells on nutrient-rich agar, inserting the desired genetic material into those cells, and then convincing the tissue to sprout into a plant. Plant biotechnology: Make it a decaf 2012-03-14T18:20:28Z To "print" the message, the researchers transferred the bacteria onto a plate containing agar, a bacterial growth medium, into which they pressed a sheet of nitrocellulose "paper" that immobilizes the bacteria. A Different Kind of Secret Code 2011-09-26T20:42:21Z The washings from the wall paper showed active development of bacteria, both in the bouillon and agar tests. Paint Technology and Tests 2011-09-15T02:00:12.263Z A bit of the culture was spread onto culture plates containing the same medium solidified with agar. From the shadows to the spotlight to the dustbin - the rise and fall of GFAJ-1 2011-06-16T13:15:02.533Z After 10−7 dilutions, we used the 5 mM AsO enrichment to inoculate an agar plate that contained the same chemical composition as the artificial medium. [Research Article] A Bacterium That Can Grow by Using Arsenic Instead of Phosphorus 2011-06-02T18:26:22.637Z Watch the spread of deforestation in Bolivia from 1984 to 1988 and compare it to bacteria on agar and you get the idea. Dot Earth: The Ultimate Invasive Species? 2011-03-01T23:36:05Z He and his graduate student Clayton Wright dyed some lab bacteria different colors, streaked it onto an agar plate in the shape of the Google logo, and let it grow. Meet the Mastermind Behind That E. Coli Google Logo 2011-02-22T21:44:36Z Ordinary agar was melted and cooled to 50° C. then mixed with about one fourth its volume of naturally sterile blood serum, and poured into sterile Petri dishes where it was allowed to solidify. Contagious Abortion of Cows 2011-02-14T03:00:33.157Z Silica gel flash chromatography was used to characterize the active ingredients in petals, and the active components were tested in agar plugs fed to beetles. Editors' Choice 2011-01-20T19:25:02.033Z An isolated colony was picked from the agar plates and reintroduced into an artificial liquid medium with no added PO, where we then progressively increased the AsO concentration to determine the optimal level for growth. [Research Article] A Bacterium That Can Grow by Using Arsenic Instead of Phosphorus 2011-06-02T18:26:22.637Z So she's trying to sculpture agar "like Jell-O" to "observe bacterial colonies in 3-D." Turning geek into chic in DIY labs 2010-12-21T01:15:04Z A typical post starts like this: "It has been a year since quick agar clarification changed my life." Cooking Issues: The Duo Behind the FCI's Food Blog 2010-09-22T09:40:00Z Nowak used ordinary agar as a medium with considerable success, and found glucose agar to be almost as favorable to the growth of the bacillus. Contagious Abortion of Cows 2011-02-14T03:00:33.157Z Solid culture media may be either permanently solid, like potatoes and coagulated egg, or they may retain their solid properties only at certain temperatures, like gelatin or agar. Outlines of dairy bacteriology A concise manual for the use of students in dairying The behaviour of agar jelly was then investigated, and the velocity of an ion through a solid jelly was shown to be very little less than in an ordinary liquid solution. Encyclopaedia Britannica, 11th Edition, Volume 6, Slice 8 "Conduction, Electric" It differs from the above by showing peculiar pink-stained granules, when stained with methylene blue and showing peculiarly convoluted chains in glucose agar. The Bacillus of Long Life a manual of the preparation and souring of milk for dietary purposes, together with and historical account of the use of fermente Whether in artificial culture a single base as gelatin or agar would suffice for all or several is yet to be discovered. The North American Slime-Moulds A Descriptive List of All Species of Myxomycetes Hitherto Reported from the Continent of North America, with Notes on Some Extra-Limital Species Each species makes a characteristic growth on gelatin, agar or other media upon which it may be cultivated. Insects and Diseases A Popular Account of the Way in Which Insects may Spread or Cause some of our Common Diseases Pick off any suspicious orange coloured colonies, plant on sloped agar, and incubate at 20° C. Observe pigment formation. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Pilar pushed his notes aside and went over to check his agar plates in the constant-temperature box. Cum Grano Salis Further, although the granule formation persists largely in milk, the organisms soon revert to the non-granular type if cultivated on agar. The Bacillus of Long Life a manual of the preparation and souring of milk for dietary purposes, together with and historical account of the use of fermente The pollen was incubated at 10° C. and at 25° C. on the agar medium for 48 and 24 hours respectively prior to making the germination counts. Northern Nut Growers Association Thirty-Fourth Annual Report 1943 For those, the Japanese seaweed called agaragar in the laboratory, but more familiarly known as agar by the layman, is excellent. Diet and Health With Key to the Calories Spread several sets of surface plates upon nutrose agar, each from one drop of the original milk sample. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Laxatives in some form are generally indicated, and one of tile best is agar- agar. Disturbances of the Heart Meanwhile, he busied himself adjusting his microscope and test-tubes and getting the agar slides ready for examination. The Silent Bullet One cubic centimeter of the resulting turbid suspension was added to 10 cc. of warm fluid agar and mixed by rotating the petri dish. Northern Nut Growers Association Thirty-Fourth Annual Report 1943 The agar may be taken two or three heaping teaspoonfuls in a large glass of water before retiring, or in the morning before breakfast, or in lieu of 4 o'clock tea. Diet and Health With Key to the Calories Pick off suspicious colonies, examine them microscopically and subcultivate upon nutrose agar in tubes; upon glucose agar and in litmus milk. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Prepare surface plate cultivations upon nutrose agar from each tube that shows growth either macroscopically or microscopically, and incubate for twenty-four hours aerobically at 37° C. 13. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Prepare gelatine and agar plates in the usual way from such of these tubes as show pellicle formation. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. In general the pollen tube grew up into the air away from the surface of the agar, rather than down into it or parallel with the surface as in normal germination. Northern Nut Growers Association Thirty-Fourth Annual Report 1943 Possibly the addition of bran, agar, or mineral oils. Diet and Health With Key to the Calories Prepare a precisely similar set of agar plates and incubate at 37° C. 6. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Prepare surface cultivations, on nutrient agar in a culture bottle, of the Bacillus mycoides, and incubate at 20° C., for forty-eight hours. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. When the filtration is completed, pour six agar plates each containing 1 c.c. of the filtrate. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Make a subcultivation from both the control and the test suspension, upon the surface of nutrient agar; incubate under optimum conditions. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Rotate the tube between the hands in order to diffuse the citrated blood evenly throughout the agar. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Number the agar tubes, consecutively, 1 to 6; the gelatine tubes, consecutively, 1 to 6, and the wort tubes, 1, 2, and 3. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Complete the solution of the various ingredients by bubbling live steam through the flask as in making nutrient agar. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Beer Wort.—Wort is chiefly used as a medium for the cultivation of yeasts, moulds, etc., both in its fluid form and also when made solid by the addition of gelatine or agar. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Weigh out powdered agar, 3 grammes; emulsify with cold distilled water, 50 c.c., and add to the contents of the flask. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Dissolve the agar and gelatine by bubbling live steam through the flask for twenty minutes. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. From the heated extract prepare duplicate sets of agar and gelatine plates and incubate anaerobically in Bulloch's apparatus at 37° C. and 20° C. respectively. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Tube in quantities of 10 c.c. and sterilise as for nutrient agar. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Treat the remaining tube of agar and serum in a similar fashion, and pour plates Nos. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Finally smear the mixture over the surface of the agar with a sterilised L-shaped rod. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Place one agar plate and one gelatine plate into the hot and cold incubators, respectively, as controls. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Pour plates of wort agar with similar quantities of the air-infected water, and incubate at 37° C. 3. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Plant tubes of gelatine or agar, liquefied by heat, from each of the higher dilutions, say from 0.0000001 loop to 0.01 loop; pour plates and incubate. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Raise the cover of the Petri dish very slightly, insert the extremity of the capillary pipette, and deposit the blood on the centre of the agar surface. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Liquefy and measure out into a sterile flask: Nutrient agar 1000 c.c. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Weigh out Lactose 10 grammes. and dissolve in the fluid agar. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Pour plates of nutrient agar with similar quantities of the water and incubate at 37° C. 4. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Measure out and mix thoroughly with the fluid agar. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Liquefy and measure out into a sterile flask Nutrient agar 1000 c.c. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Weigh out agar 20 grammes and emulsify in 100 c.c. cold distilled water. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Dissolve the agar by bubbling live steam through the flask. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Pour the contents of a tube of liquefied agar—not inoculated—into a Petri dish to act as a control to demonstrate the sterility of the batch of agar employed. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Weigh out 15 grammes agar, emulsify in a separate vessel with 200 c.c. of the alkaline fluid previously cooled to about 20°C., and then add to the remainder of the fluid in the flask. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. If desired, add glycerine, 5 per cent., to the clear agar. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Note.—Gelatine and agar oblique tubes should be freshly "slanted" before use. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Raise the agar block on the blade of the scalpel and transfer it, under side down, to the centre of a sterile slide. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Count and record the number of colonies that have developed upon the agar at 37° C. after forty-eight hours' incubation. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Invert the preparation and with the blade of the scalpel remove the slide from the agar block. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Cool to 60° C. and clarify with egg as for nutrient agar. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Weigh out: lactose 10 grammes and dissolve in the fluid agar. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Deliver a small quantity of the blood on the slanted surface of the agar in each of the tubes, and allow it to run over the entire surface of the medium. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Pick off streptococcus colonies and subcultivate upon nutrient agar exactly as directed in steps 14, 15 and 16. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Prepare a surface cultivation on nutrient agar in a culture bottle and incubate under optimum conditions for forty-eight hours. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Weigh out 15 grammes agar and mix into a thick paste with 100 c.c. cold distilled water, and add to the flask. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Dissolve the agar by bubbling live steam through the mixture as in making nutrient agar. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Dissolve the agar by passing live steam through it, as in preparing nutrient agar. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. If the resulting growth shows colonies which resemble those of the streptococcus, make subcultivations upon agar and in bouillon, in the first instance, and study carefully. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Weigh out dextrose 20 grammes and dissolve in the fluid agar. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. If, however, it is to be used as the basis for agar or gelatine media store it in the "Double Strength" condition. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Bubble live steam through the mixture to dissolve the gelatine and agar. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Bubble live steam through the mixture for twenty minutes, to dissolve the agar. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. If the resulting growth resembles that of the streptococcus, make subcultivations upon nutrient agar. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Dissolve the agar and gelatine by bubbling live steam through the flask for twenty minutes. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Bubble live steam through the mixture for twenty minutes, to dissolve the agar. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Liquefy and measure out into a sterile flask Nutrient agar 1000 c.c. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Liquefy and measure out into a sterile flask Nutrient agar 1000 c.c. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Inoculate an agar slope and a broth tube from the emulsion in the stock dish corresponding to each of these specially selected numbers. b. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Weigh out powdered agar, 20 grammes; mix into a smooth paste with 200 c.c. of cold wort and add to the wort in the flask. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Bubble live steam through the mixture for twenty minutes to dissolve the agar. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Filter directly into the flask containing the melted agar mixture. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Weigh out Dextrose 10 grammes. and dissolve in nutrient agar. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Prepare cover-slip films from the agar cultures, after twenty-four hours' incubation. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Liquefy and measure out into a sterile flask Nutrient agar 1000 c.c. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Weigh out and dissolve in the fluid agar. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. With a sterile 10 c.c. graduated pipette transfer 1 c.c. citrated blood from the Erlenmeyer flask to each tube of liquefied agar. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Clarify with egg, and filter as for nutrient agar. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Melt the gelatine and the agar tubes in boiling water; then transfer to the water-bath and cool them down to 42° C. 7. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Weigh out powdered agar, 13 grammes, and emulsify with 200 c.c. cold distilled water. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Weigh out French maltose, 40 grammes, and dissolve in the agar. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Weigh out powdered agar, 5 grammes, emulsify with 100 c.c., cold distilled water and add to the contents of the flask. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Cool to 60° C. Clarify with egg as for nutrient agar. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Pour plates from the agar tubes; label, and incubate at 37° C. 14. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Cool to 60° C. Clarify with egg as for nutrient agar. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. In order that the medium may adhere firmly to the glass, the agar used for roll cultivation should have 1 per cent. gelatine or 1 per cent. gum arabic added to it before sterilisation. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Liquefy three tubes of nutrient agar—nutrose agar or the like. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Tubes of broth, or liquefied gelatine or agar. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. After twenty-four hours' incubation "inspect," and after forty-eight hours' incubation, "count" the agar plates and estimate the number of "organisms growing at 37° C." present per cubic centimetre of the sample of milk. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. The average weight of a measured litre of 2 per cent. agar when prepared in this way, after filtration, is 1010.5 grammes. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Liquefy and measure out into a sterile flask: Nutrient agar 1000 c.c. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. With a sharp scalpel cut out a block some 8 mm. square, from the entire thickness of the agar layer. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. With a platinum loop run a drop or two of melted agar around the edges of the block. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Examine carefully the growth upon the serial blood serum cultivations prepared to isolate B. diphtheriæ and the serial agar cultivations to isolate streptococci after forty-eight hours' incubation. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Weigh out 250 grammes haricot beans, place in the flask with the agar mixture. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Weigh out 10 grammes agar and make into a thick paste with 100 c.c. cold distilled water. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Add this agar paste to the distilled water in the flask. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Litmus Whey Agar is prepared in a similar manner to Whey Gelatine, with the substitution of 15 grammes of agar for the gelatine. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Pour similar sets of agar plates from the contents of capsules II and III; label and incubate at 37° C. 14. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Bubble live steam through the mixture for twenty minutes to dissolve the agar. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Return the "blood agar" to the hot incubator for forty-eight hours and eliminate any contaminated tubes. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. From the same tubes pour agar plates and incubate anaerobically in Bulloch's apparatus, at 37° C. 5. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Subcultivate suspicious colonies on the various media, incubate anaerobically, making control cultivations on glucose formate agar, stab and streak, to incubate aerobically and carry out further inoculation experiments with the resulting growths. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Make a stab culture in gelatine or agar, choosing for the purpose a straight tube containing a deep column of medium, and thrusting the inoculating needle to the bottom of the tube. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Standardising Nutrient Agar and Gelatine.—The method of standardising agar and gelatine is precisely similar to that described under bouillon. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. If badly "egged," and also during the winter months, it is necessary to surround the glass funnel, in which the filtration of the agar is carried on, by a hot-water jacket. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Heat in the steamer at 100° C. for ninety minutes to dissolve the agar. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Weigh out powdered agar, 5 grammes, and emulsify it with 200 c.c. double strength fish bouillon. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Pour twenty plate cultivations, ten of nutrient gelatine and ten of nutrient agar, each containing 0.1 c.c. of the bouillon culture. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Method.—The cultivations employed should be smear agar cultures, twelve to eighteen hours old if incubated at 37°C, twenty-four to thirty hours if incubated at 22°C. 1. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Make hanging-drop preparations from the bouillon and agar cultivations after twenty-four hours' incubation. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. Melt three tubes of nutrient agar in boiling water and cool to 42° C. 5. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. For any particular experiment prepare a smear culture on agar and incubate at 37° C. for 24 hours anaerobically. The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. |
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