Furthermore, the protein isolated in the periplasm can be purified by nickel ion affinity chromatography.

而且从周质空间分离的蛋白经过镍住得到了进一步的纯化。

Methods A combination of euglobulin precipitation, ion-exchange chromatography,(NH4)2SO4 precipitation and affinity chromatography was used in the process of purification.

方法采用优球蛋白沉淀、离子交换色谱、(NH4)2SO4沉淀和亲和色谱法进行纯化。