Methods The sciatic and brachial plexus nerve of three-day-old SD mice was treated with collagenase type IV and trypsin to remove the desmocyte on the Schwann cell, then explanted in the culture disk.

先用IV型胶原酶和胰蛋白酶消化出生3天的SD鼠臂层神经和坐骨神经组织块后再将组织块贴壁培养。